Controlled Expression and Functional Analysis of the Iron-Sulfur Cluster (Isc) Biosynthetic Machinery in Azotobacter vinelandii
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چکیده
........................................................................................ii ACKNOWLEDGEMENTS.......................................................................iv TABLE OF CONTENTS..........................................................................v LIST OF FIGURES................................................................................vii LIST OF TABLES..................................................................................x CHAPTER 1: Introduction to [Fe-S] Cluster Biosynthesis: the requirement of a controlled expression system in A. vinelandii...................................1 CHAPTER 2: Literature Review: Structure, Function, and Formation of Biological Iron-Sulfur Clusters.....................................................................5 2.
منابع مشابه
Controlled expression and functional analysis of iron-sulfur cluster biosynthetic components within Azotobacter vinelandii.
A system for the controlled expression of genes in Azotobacter vinelandii by using genomic fusions to the sucrose catabolic regulon was developed. This system was used for the functional analysis of the A. vinelandii isc genes, whose products are involved in the maturation of [Fe-S] proteins. For this analysis, the scrX gene, contained within the sucrose catabolic regulon, was replaced by the c...
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In recent years, it has become evident that [Fe-S] proteins, such as hydrogenase, nitrogenase and aconitase, require a complex machinery to assemble and insert their associated [Fe-S] clusters. So far, three different types of [Fe-S] cluster biosynthetic systems have been identified and these have been designated nif, isc and suf. In the present work, we show that the nif-specific [Fe-S] cluste...
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